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KMID : 0578320120340050463
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Molecules and Cells
2012 Volume.34 No. 5 p.463 ~ p.471
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Constitutive Expression of Mammalian Nitric Oxide Synthase in Tobacco Plants Triggers Disease Resistance to Pathogens
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Chun Hyun-Jin
Park Hyeong-Cheol
Koo Sung-Cheol
Lee Ju-Huck
Park Chan-Young
Choi Man-Soo
Kang Chang-Ho
Baek Dong-Won
Cheong Yong-Hwa
Yun Dae-Jin
Chung Woo-Sik
Cho Moo-Je
Kim Min-Chul
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Abstract
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Nitric oxide (NO) is known for its role in the activation of plant defense responses. To examine the involvement and mode of action of NO in plant defense responses, we introduced calmodulin-dependent mammalian neuronal nitric oxide synthase (nNOS), which controls the CaMV35S promoter, into wild-type and NahG tobacco plants. Constitutive expression of nNOS led to NO production and triggered spontaneous induction of leaf lesions. Transgenic plants accumulated high amounts of H2O2, with catalase activity lower than that in the wild type. nNOS transgenic plants contained high levels of salicylic acid (SA), and they induced an array of SA-, jasmonic acid (JA)-, and/or ethylene (ET)-related genes. Consequently, NahG co-expres-sion blocked the induction of systemic acquired resistance (SAR)-associated genes in transgenic plants, implying SA is involved in NO-mediated induction of SAR genes. The transgenic plants exhibited enhanced resistance to a spectrum of pathogens, including bacteria, fungi, and viruses. Our results suggest a highly ranked regulatory role for NO in SA-, JA-, and/or ET-dependent pathways that lead to disease resistance.
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KEYWORD
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nitric oxide (NO), nitric oxide synthase (NOS), plant defense signaling, reactive oxygen species, salicylic acid
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