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KMID : 0578320120340060577
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Molecules and Cells
2012 Volume.34 No. 6 p.577 ~ p.583
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Identification of a Novel Cell-Penetrating Peptide from Human Phosphatidate Phosphatase LPIN3
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Lim Sang-Ho
Kim Won-Ju
Kim Yeon-Ho
Choi Je-Min
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Abstract
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Biomolecules such as proteins, DNA, and RNA are mac-romolecules and can not cross the cell membrane. How-ever, cell-penetrating peptide (CPP) has been shown to deliver therapeutic biomolecules successfully into cells. The various and widely used CPPs including TAT, VP22, and Antp are mostly non-human originated CPPs, and are limited by their potential toxicity and immunogenicity. We report here on a newly identified novel cell-penetrating sequence (LPIN; RRKRRRRRK) from the nuclear localization sequence (NLS) of human nuclear phosphatase, LPIN3. LPIN-EGFP recombinant protein was concentration- and time-dependently delivered into cells and localized to the nucleus as well as the cytoplasm. It penetrated the cell membrane by lipid raft-mediated endocytosis by binding to heparan sulfate proteoglycan. LPIN-EGFP was successfully delivered into primary mouse splenocytes in vitro and it could be delivered into various tissues including liver, kidney, and intestine in mice after intra-peritoneal injection. This re-search suggests that LPIN-CPP could be used in a drug delivery system to deliver therapeutic biomolecules including peptides, proteins, DNA, and RNA and without the limitations of non-human originated CPPs such as TAT-CPP.
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KEYWORD
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CPP, drug delivery system, LPIN, PTD
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