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KMID : 0578320130350040320
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Molecules and Cells
2013 Volume.35 No. 4 p.320 ~ p.326
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RNA Interference-Mediated Simultaneous Silencing of Four Genes Using Cross-Shaped RNA
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Lee Tae-Yeon
Chang Chan-Il
Lee Doo-Young
Hong Sun-Woo
Shin Chan-Seok
Chiang J. Li
Kim So-Youn
Dirk Haussecker
Lee Dong-Ki
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Abstract
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The structural flexibility of RNA interference (RNAi)-triggering nucleic acids suggests that the design of unconventional RNAi trigger structures with novel features is possible. Here, we report a cross-shaped RNA duplex structure, termed quadruple interfering RNA (qiRNA), with multiple target gene silencing activity. qiRNA triggers the simultaneous down-regulation of four cellular target genes via an RNAi mechanism. In addition, qiRNA shows enhanced intracellular delivery and target gene silencing over conventional siRNA when complexed with jetPEI, a linear polyethyleneimine (PEI). We also show that the long antisense strand of qiRNA is incorporated intact into an RNA-induced silencing complex (RISC). This novel RNA scaffold further expands the repertoire of RNAi-triggering molecular structures and could be used in the development of therapeutics for various diseases including viral infections and cancer.
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KEYWORD
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polyethyleneimine (PEI), quadruple interfering RNA (qiRNA), RNA interference, small interfering RNA (siRNA)
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