KMID : 0578320130350060519
|
|
Molecules and Cells 2013 Volume.35 No. 6 p.519 ~ p.525
|
|
Fruit Indehiscence Caused by Enhanced Expression of NO TRANSMITTING TRACT in Arabidopsis thaliana
|
|
Chung Kyung-Sook
Lee Jeong-Hwan Lee Jong-Seob Ahn Ji-Hoon
|
|
Abstract
|
|
|
In flowering plants, fruit dehiscence enables seed dispersal. Here we report that ntt-3D, an activation tagged allele of NO TRANSMITTING TRACT (NTT), caused a failure of fruit dehiscence in Arabidopsis. We identified ntt-3D, in which the 35S enhancer was inserted adjacent to AT3G-57670, from our activation tagged mutant library. ntt-3D mutants showed serrated leaves, short siliques, and indehiscence phenotypes. NTT-overexpressing plants largely phenocopied the ntt-3D plants. As the proximate cause of the indehiscence, ntt-3D plants exhibited a near absence of valve margin and lignified endocarp b layer in the carpel. In addition, the replum was enlarged in ntt-3D mutants. NTT expression reached a peak in flowers at stage 11 and gradually decreased thereafter and pNTT::GUS expression was mainly observed in the replum, indicating a potential role in fruit patterning. NTT:GFP localized in the nucleus and cytoplasm. FRUITFULL (FUL) expression was downregulated in ntt-3D mutants and ntt-3D suppressed upregulation of FUL in replumless mutants. These results indicate that NTT suppresses FUL, indicating a potential role in patterning of the silique. In seed crops, a reduction in pod dehiscence can increase yield by decreasing seed dispersal; therefore, our results may prove useful as a basis to improve crop yield.
|
|
KEYWORD
|
|
carpel development, dehiscent zone, endocarp b layer, fruit indehiscence, replum
|
|
FullTexts / Linksout information
|
|
|
|
Listed journal information
|
|
|
|