KMID : 0578320130360060507
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Molecules and Cells 2013 Volume.36 No. 6 p.507 ~ p.517
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Identification of Nucleolin as a Lipid-Raft-Dependent ¥â1-Integrin-Interacting Protein in A375 Cell Migration
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Jiajia Bi
Ruifei Wang Yue Zhang Xiaoqing Han Khamal Kwesi Ampah Xianlu Zeng
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Abstract
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Lipid rafts are related to cell surface receptor function. Integrin is a major surface receptor protein in cell adhesion and migration on the extracellular matrix (ECM). Here, we showed that lipid rafts played a critical role in human melanoma A375 cell spreading and migration on fibronectin; an important component of the ECM that interacts with ¥â1 integrin. We found that the disruption of lipid rafts did not markedly inhibit the expression and activation of ¥â1 integrin. By coimmunoprecipitation and mass spectrometry, we investigated the influence of lipid rafts on the ¥â1 integrin complex and identified nucleolin as a potential lipid-raft-dependent ¥â1-integrin-interacting protein. Upon confirmation of the interaction between ¥â1 integrin and nucleolin, further studies revealed that nucleolin colocalized with ¥â1 integrin in lipid rafts and raft disruption interrupted their association. In addition, knockdown of nucleolin markedly attenuated A375 cell spreading and migration on fibronectin. Taken together, we demonstrated that nucleolin is a critical lipid-raft-dependent ¥â1-integrin-interacting protein in A375 cell spreading and migration on fibronectin.
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KEYWORD
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¥â1 integrin, lipid rafts, mass spectrometry, melanoma cell spreading and migration, nucleolin
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