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KMID : 0578320140370010031
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Molecules and Cells
2014 Volume.37 No. 1 p.31 ~ p.35
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Ground-State Conditions Promote Robust Prdm14 Reactivation and Maintain an Active Dlk1-Dio3 Region during Reprogramming
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Omer Habib
Gizem Habib
Moon Sung-Hwan
Hong Ki-Sung
Do Jeong-Tae
Choi Young-Sok
Chang Sung-Woon
Chung Hyung-Min
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Abstract
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Induced pluripotent stem cells (iPSCs) are capable of unlimited self-renewal and can give rise to all three germ layers, thereby providing a new platform with which to study mammalian development and epigenetic reprogramming. However, iPSC generation may result in subtle epigenetic variations, such as the aberrant methylation of the Dlk1-Dio3 locus, among the clones, and this heterogeneity constitutes a major drawback to harnessing the full potential of iPSCs. Vitamin C has recently emerged as a safeguard to ensure the normal imprinting of the Dlk1-Dio3 locus during reprogramming. Here, we show that vitamin C exerts its effect in a manner that is independent of the reprogramming kinetics. Moreover, we demonstrate that reprogramming cells under 2i conditions leads to the early upregulation of Prdm14, which in turn results in a highly homogeneous population of authentic pluripotent colonies and prevents the abnormal silencing of the Dlk1-Dio3 locus.
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KEYWORD
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2i conditions, Dlk1-Dio3, induced pluripotent stem cells, Prdm14
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