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KMID : 0578320140370010043
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Molecules and Cells
2014 Volume.37 No. 1 p.43 ~ p.50
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HIF-1-Dependent Induction of Jumonji Domain-Containing Protein (JMJD) 3 under Hypoxic Conditions
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Lee Ho-Youl
Choi Kang
Oh Hoo-Keun
Park Young-Kwon
Park Hyun-Sung
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Abstract
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Jumonji domain-containing proteins (JMJD) catalyze the oxidative demethylation of a methylated lysine residue of histones by using O, α-ketoglutarate, vitamin C, and Fe(II). Several JMJDs are induced by hypoxic stress to compensate their presumed reduction in catalytic activity under hypoxia. In this study, we showed that an H3K27me3 specific histone demethylase, JMJD3 was induced by hypoxia-inducible factor (HIF)-1α/β under hypoxia and that treatment with Clioquinol, a HIF-1α activator, increased JMJD3 expression even under normoxia. Chromatin immunoprecipitation (ChIP) analyses showed that both HIF-1α and its dimerization partner HIF-1β/Arnt occupied the first intron region of the mouse gene, whereas the HIF-1α/β heterodimer bound to the upstream region of the human , indicating that human and mouse have hypoxia-responsive regulatory regions in different locations. This study shows that both mouse and human are induced by HIF-1.
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KEYWORD
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Arnt, JMJD3, HIF-1α, histone demethylase, hypoxia
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