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KMID : 0578320140370010074
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Molecules and Cells
2014 Volume.37 No. 1 p.74 ~ p.80
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Developmental Roles of D-bifunctional Protein-A Zebrafish Model of Peroxisome Dysfunction
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Kim Yong-Il
Sushil Bhandari
Lee Joon-No
Yoo Kyeong-Won
Kim Se-Jin
Oh Gi-Su
Kim Hyung-Jin
Cho Me-Young
Kwak Jong-Young
So Hong-Seob
Park Rae-Kil
Choe Seong-Kyu
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Abstract
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The peroxisome is an intracellular organelle that responds dynamically to environmental changes. Various model organisms have been used to study the roles of peroxisomal proteins in maintaining cellular homeostasis. By taking advantage of the zebrafish model whose early stage of embryogenesis is dependent on yolk components, we examined the developmental roles of the D-bifunctional protein (Dbp), an essential enzyme in the peroxisomal β-oxidation. The knockdown of in zebrafish phenocopied clinical manifestations of its deficiency in human, including defective craniofacial morphogenesis, growth retardation, and abnormal neuronal development. Overexpression of murine rescued the morphological phenotypes induced by knockdown, indicative of conserved roles of Dbp during zebrafish and mammalian development. Knockdown of impaired normal development of blood, blood vessels, and most strikingly, endoderm-derived organs including the liver and pancreas - a phenotype not reported elsewhere in connection with peroxisome dysfunction. Taken together, our results demonstrate for the first time that zebrafish might be a useful model animal to study the role of peroxisomes during vertebrate development.
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KEYWORD
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D-bifunctional protein, embryogenesis, model organism, peroxisome, zebrafish
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