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KMID : 0578320140370040314
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Molecules and Cells
2014 Volume.37 No. 4 p.314 ~ p.321
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Inhibition of Cell Proliferation and Migration by miR-509-3p That Targets CDK2, Rac1, and PIK3C2A
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Yoon Se-Na
Han Eun-Ji
Choi Young-Chul
Kee Hong-Hwan
Jeong Yong-Su
Yoon Jae-Seung
Baek Kwang-Hee
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Abstract
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CDK2 is a key regulator of cell cycle progression. In this study, we screened for miRNAs targeting CDK2 using a luciferase-3′-untranslated region reporter assay. Among 11 hit miRNAs, miR-509-3p reduced CDK2 protein levels and significantly inhibited cancer cell growth. Microarray, Western blotting, and luciferase reporter analyses revealed additional targets of miR-509-3p, including Rac1 and PIK3C2A. Overexpression of miR-509-3p induced G1 cell-cycle arrest and inhibited colony formation and migration. RNAi experiments indicated that the growth-inhibitory effects of miR-509-3p may occur through down-regulation of CDK2, Rac1, and PIK3C2A. Targeting of multiple growth regulatory genes by miR-509-3p may contribute to effective anti-cancer therapy.
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KEYWORD
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CDK2, growth inhibition, microRNA, miR-509-3p, PIK3C2A, Rac1
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