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KMID : 0578320140370050418
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Molecules and Cells
2014 Volume.37 No. 5 p.418 ~ p.425
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Increase in Hypotonic Stress-Induced Endocytic Activity in Macrophages via ClC-3
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Yan Yu Tao
Ding Yu
Ming Bing Xia
Du Wen Jiao
Kong Xiao Ling
Tian Li
Zheng Fang
Fang Min
Tan Zheng
Gong Fei Li
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Abstract
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Extracellular hypotonic stress can affect cellular function. Whether and how hypotonicity affects immune cell function remains to be elucidated. Macrophages are immune cells that play key roles in adaptive and innate in immune reactions. The purpose of this study was to investigate the role and underlying mechanism of hypotonic stress in the function of bone marrow-derived macrophages (BMDMs). Hypotonic stress increased endocytic activity in BMDMs, but there was no significant change in the expression of CD80, CD86, and MHC class II molecules, nor in the secretion of TNF-α or IL-10 by BMDMs. Furthermore, the enhanced endocytic activity of BMDMs triggered by hypotonic stress was significantly inhibited by chloride channel-3 (ClC-3) siRNA. Our findings suggest that hypotonic stress can induce endocytosis in BMDMs and that ClC-3 plays a central role in the endocytic process.
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KEYWORD
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chloride channels -3, endocytosis, hypotonic stress, macrophage
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