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KMID : 0578320140370100742
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Molecules and Cells
2014 Volume.37 No. 10 p.742 ~ p.746
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Identification of DNA Aptamers toward Epithelial Cell Adhesion Molecule via Cell-SELEX
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Kim Ji-Won
Kim Eun-Young
Kim Sun-Young
Byun Sang-Kyung
Lee Da-Som
Oh Kyoung-Jin
Kim Won-Kon
Han Baek-Soo
Chi Seung-Wook
Lee Sang-Chul
Bae Kwang-Hee
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Abstract
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The epithelial cell adhesion molecule (EpCAM, also known as CD326) is a transmembrane glycoprotein that is specifically detected in most adenocarcinomas and cancer stem cells. In this study, we performed a Cell systematic evolution of ligands by exponential enrichment (SELEX) experiment to isolate the aptamers against EpCAM. After seven round of Cell SELEX, we identified several aptamer candidates. Among the selected aptamers, EP166 specifically binds to cells expressing EpCAM with an equilibrium dissociation constant (Kd) in a micromolar range. On the other hand, it did not bind to negative control cells. Moreover, EP166 binds to J1ES cells, a mouse embryonic stem cell line. Therefore, the isolated aptamers against EpCAM could be used as a stem cell marker or in other applications in both stem cell and cancer studies.
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KEYWORD
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aptamer, EpCAM, SELEX, stem cell marker
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