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KMID : 0578320150380010051
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Molecules and Cells
2015 Volume.38 No. 1 p.51 ~ p.57
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A Role for Peroxidasin PXN-1 in Aspects of C. elegans Development
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Lee Ju-Yeon
Bandyopadhyay Jaya
Lee Jin-Il
Cho In-Jeong
Park Dae-Ho
Cho Jeong-Hoon
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Abstract
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The Caenorhabditis elegans peroxidasins, PXN-1 and PXN-2, are extracellular peroxidases; pxn-2 is involved in muscle-epidermal attachment during embryonic morphogenesis and in specific axon guidance. Here we investigate potential roles of the other homologue of peroxidasin, pxn-1, in C. elegans. A pxn-1 deletion mutant showed high lethality under heat-stress conditions. Using a transcriptional GFP reporter, pxn-1 expression was observed in various tissues including neurons, muscles, and hypodermis. A translational fusion showed that PXN-1::GFP was secreted and localized in extracellular matrix, particularly along body wall muscles and pharyngeal muscles. Various neuronal developmental defects were observed in pxn-1 mutants and in pxn-1 over-expressing animals, including handedness, branching, breakage, tangling, and defasciculation. These results suggest that pxn-1, like other peroxidasins, plays an important role throughout development.
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KEYWORD
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C. elegans, heat stress, neuronal development, peroxidasins, pxn-1
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