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KMID : 0578320150380010065
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Molecules and Cells
2015 Volume.38 No. 1 p.65 ~ p.74
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Comparative N-Linked Glycan Analysis of Wild-Type and α1,3-Galactosyltransferase Gene Knock-Out Pig Fibroblasts Using Mass Spectrometry Approaches
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Park Hae-Min
Kim Yoon-Woo
Kim Kyoung-Jin
Kim Young-June
Yang Yung-Hun
Jin Jang-Mi
Kim Young-Hwan
Kim Byung-Gee
Shim Ho-Sup
Kim Yun-Gon
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Abstract
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Carbohydrate antigens expressed on pig cells are considered to be major barriers in pig-to-human xenotransplantation. Even after α1,3-galactosyltransferase gene knock-out (GalT-KO) pigs are generated, potential non-Gal antigens are still existed. However, to the best of our knowledge there is no extensive study analyzing N-glycans expressed on the GalT-KO pig tissues or cells. Here, we identified and quantified totally 47 N-glycans from wild-type (WT) and GalT-KO pig fibroblasts using mass spectrometry. First, our results confirmed the absence of galactose-alpha-1,3-galactose (α-Gal) residue in the GalT-KO pig cells. Interestingly, we showed that the level of overall fucosylated N-glycans from GalT-KO pig fibroblasts is much higher than from WT pig fibroblasts. Moreover, the relative quantity of the N-glycolylneuraminic acid (NeuGc) antigen is slightly higher in the GalT-KO pigs. Thus, this study will contribute to a better understanding of cellular glycan alterations on GalT-KO pigs for successful xenotransplantation.
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KEYWORD
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GalT-KO pig fibroblast, mass spectrometry (MS), N-glycan, N-glycolylneuraminic acid (NeuGc)
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