KMID : 0578320150380020112
|
|
Molecules and Cells 2015 Volume.38 No. 2 p.112 ~ p.121
|
|
14-3-3¥æ Regulates Immune Response through Stat3 Signaling in Oral Squamous Cell Carcinoma
|
|
Han Xin Guang
Han Yong Fu Jiao Hui Feng Jie Ya Qiong
|
|
Abstract
|
|
|
Ectopic expression of 14-3-3¥æ has been found in various malignancies, including lung cancer, liver cancer, head and neck squamous cell carcinoma (HNSCC), and so on. However, the effect of 14-3-3¥æ in the regulation of interactions between tumor cells and the immune system has not been previously reported. In this study, we aimed to investigate whether and how 14-3-3¥æ is implicated in tumor inflammation modulation and immune recognition evasion. In oral squamous cell carcinoma (OSCC) cell lines and cancer tissues, we found that 14-3-3¥æ is overexpressed. In OSCC cells, 14-3-3¥æ knockdown resulted in the up-regulated expression of inflammatory cytokines. In contrast, 14-3-3¥æ introduction attenuated cytokine expression in human normal keratinocytes and fibroblasts stimulated with interferon-¥ã (IFN-¥ã) and lipopolysaccharide (LPS). Furthermore, supernatants from 14-3-3¥æ knockdown OSCC cells dramatically altered the response of peritoneal macrophages, dendritic cells and tumor-specific T cells. Interestingly, Stat3 was found to directly interact with 14-3-3¥æ and its disruption relieved the inhibition induced by 14-3-3¥æ in tumor inflammation. Taken together, our studies provide evidence that 14-3-3¥æ may regulate tumor inflammation and immune response through Stat3 signaling in OSCC.
|
|
KEYWORD
|
|
14-3-3¥æ, immune response, oral squamous cell carcinoma, Stat3, tumor inflammation
|
|
FullTexts / Linksout information
|
|
|
|
Listed journal information
|
|
|
|