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KMID : 0578320150380030210
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Molecules and Cells
2015 Volume.38 No. 3 p.210 ~ p.220
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Genome-Wide Analysis of DNA Methylation before-and after Exercise in the Thoroughbred Horse with MeDIP-Seq
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Gim Jeong-An
Hong Chang-Pyo
Kim Dae-Soo
Moon Jae-Woo
Choi Yu-Ri
Eo Jung-Woo
Kwon Yun-Jeong
Lee Ja-Rang
Jung Yi-Deun
Bae Jin-Han
Choi Bong-Hwan
Ko Jun-Su
Song Sang-Hoon
Ahn Kung
Ha Hong-Seok
Yang Young-Mok
Lee Hak-Kyo
Park Kyung-Do
Do Kyoung-Tag
Han Kyu-Dong
Yi Joo-Mi
Cha Hee-Jae
Ayarpadikannan Selvam
Cho Byung-Wook
Bhak Jong
Kim Heui-Soo
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Abstract
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Athletic performance is an important criteria used for the selection of superior horses. However, little is known about exercise-related epigenetic processes in the horse. DNA methylation is a key mechanism for regulating gene expression in response to environmental changes. We carried out comparative genomic analysis of genome-wide DNA methylation profiles in the blood samples of two different thoroughbred horses before and after exercise by methylated-DNA immunoprecipitation sequencing (MeDIP-Seq). Differentially methylated regions (DMRs) in the pre-and post-exercise blood samples of superior and inferior horses were identified. Exercise altered the methylation patterns. After 30 min of exercise, 596 genes were hypomethylated and 715 genes were hypermethylated in the superior horse, whereas in the inferior horse, 868 genes were hypomethylated and 794 genes were hypermethylated. These genes were analyzed based on gene ontology (GO) annotations and the exercise-related pathway patterns in the two horses were compared. After exercise, gene regions related to cell division and adhesion were hypermethylated in the superior horse, whereas regions related to cell signaling and transport were hypermethylated in the inferior horse. Analysis of the distribution of methylated CpG islands confirmed the hypomethylation in the gene-body methylation regions after exercise. The methylation patterns of transposable elements also changed after exercise. Long interspersed nuclear elements (LINEs) showed abundance of DMRs. Collectively, our results serve as a basis to study exercise-based reprogramming of epigenetic traits.
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KEYWORD
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DNA methylation, exercise, MeDIP-Seq, thoroughbred horse, transposable elements
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