|
KMID : 0578320150380040304
|
|
Molecules and Cells
2015 Volume.38 No. 4 p.304 ~ p.311
|
|
|
Expression and Preliminary Functional Profiling of the let-7 Family during Porcine Ovary Follicle Atresia
|
|
Cao Rui
Wu Wang Jun
Zhou Xiao Long
Xiao Peng
Wang Yi
Liu Hong Lin
|
|
|
Abstract
|
|
|
|
Most follicles in the mammalian ovary undergo atresia. Granulosa cell apoptosis is a hallmark of follicle atresia. Our previous study using a microRNA (miRNA) microarray showed that the let-7 microRNA family was differentially expressed during follicular atresia. However, whether the let-7 miRNA family members are related to porcine (Sus scrofa) ovary follicular apoptosis is unclear. In the current study, real-time quantitative polymerase chain reaction showed that the expression levels of let-7 family members in follicles and granulosa cells were similar to our microarray data, in which miRNAs let-7a, let-7b, let-7c, and let-7i were significantly decreased in early atretic and progressively atretic porcine ovary follicles compared with healthy follicles, while let-7g was highly expressed during follicle atresia. Furthermore, flow cytometric analysis and Hoechst33342 staining demonstrated that let-7g increased the apoptotic rate of cultured granulosa cells. In addition, let-7 target genes were predicted and annotated by TargetScan, PicTar, gene ontology and Kyoto encyclopedia of genes and genomes pathways. Our data provide new insight into the association between the let-7 miRNA family in granulosa cell programmed death.
|
|
|
KEYWORD
|
|
|
apoptosis, follicle atresia, let-7, MicroRNA, MiRNA, pig, stem-loop
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|
|