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KMID : 0578320150380050396
Molecules and Cells
2015 Volume.38 No. 5 p.396 ~ p.401
3¡Ç,4¡Ç,5¡Ç,5,7-Pentamethoxyflavone Sensitizes Cisplatin-Resistant A549 Cells to Cisplatin by Inhibition of Nrf2 Pathway
Hou Xiang Yu

Bai Xu Peng
Gou Xiao Li
Zeng Hang
Xia Chen
Zhuang Wei
Chen Xin Meng
Zhao Zhong Xiang
Huang Min
Jin Jing
Abstract
Nuclear factor erythroid 2-related factor 2 (Nrf2) is an important redox-sensitive transcription factor that regulates the expression of several cytoprotective genes. More recently, genetic analyses of human tumors have indicated that Nrf2 may cause resistance to chemotherapy. In this study, we found that the expression levels of Nrf2 and its target genes GCLC, HO-1, NQO1 were significantly higher in cisplatin-resistant A549 (A549/CDDP) cells than those in A549 cells, and this resistance was partially reversed by Nrf2 siRNA. 3¡Ç,4¡Ç,5¡Ç,5,7-Pentamethoxyflavone (PMF), a natural flavonoid extracted from Rutaceae plants, sensitized A549/CDDP to CDDP and substantially induced apoptosis compared with that of CDDP alone treated group, and this reversal effect decreased when Nrf2 was downregulated by siRNA. Mechanistically, PMF reduced Nrf2 expression leading to a reduction of Nrf2 downstream genes, and in contrast, this effect was decreased by blocking Nrf2 with siRNA. Taken together, these results demonstrated that PMF could be used as an effective adjuvant sensitizer to increase the efficacy of chemotherapeutic drugs by downregulating Nrf2 signaling pathway.
KEYWORD
3¡Ç,4¡Ç,5¡Ç,5,7-Pentamethoxyflavone, chemoresistance, cisplatin, lung cancer, Nrf2
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