KMID : 0578320160390020163
|
|
Molecules and Cells 2016 Volume.39 No. 2 p.163 ~ p.168
|
|
Caffeine Induces the Stress Response and Up-Regulates Heat Shock Proteins in Caenorhabditis elegans
|
|
Al Amin Mohammad
Kawasaki Ichiro Gong Joo-Mi Shim Yhong-Hee
|
|
Abstract
|
|
|
Caffeine has both positive and negative effects on physiological functions in a dose-dependent manner. C. elegans has been used as an animal model to investigate the effects of caffeine on development. Caffeine treatment at a high dose (30 mM) showed detrimental effects and caused early larval arrest. We performed a comparative proteomic analysis to investigate the mode of action of high-dose caffeine treatment in C. elegans and found that the stress response proteins, heat shock protein (HSP)-4 (endoplasmic reticulum [ER] chaperone), HSP-6 (mitochondrial chaperone), and HSP-16 (cytosolic chaperone), were induced and their expression was regulated at the transcriptional level. These findings suggest that high-dose caffeine intake causes a strong stress response and activates all three stress-response pathways in the worms, including the ER-, mitochondrial-, and cytosolic pathways. RNA interference of each hsp gene or in triple combination retarded growth. In addition, caffeine treatment stimulated a food-avoidance behavior (aversion phenotype), which was enhanced by RNAi depletion of the hsp-4 gene. Therefore, up-regulation of hsp genes after caffeine treatment appeared to be the major responses to alleviate stress and protect against developmental arrest.
|
|
KEYWORD
|
|
caffeine, C. elegans development, food-avoidance behavior, heat shock protein, proteomic analysis
|
|
FullTexts / Linksout information
|
|
|
|
Listed journal information
|
|
|
|