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KMID : 0578320160390100756
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Molecules and Cells
2016 Volume.39 No. 10 p.756 ~ p.761
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Carboxypeptidase E, Identified As a Direct Interactor of Growth Hormone, Is Important for Efficient Secretion of the Hormone
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Mizutani Akiko
Inoko Hidetoshi
Tanaka Masafumi
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Abstract
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We have identified 88 interactor candidates for human growth hormone (GH) by the yeast two-hybrid assay. Among those, we focused our efforts on carboxypeptidase E (CPE), which has been thought to play a key role in sorting prohormones, such as pro-opiomelanocortin (POMC), to regulated secretory vesicles. We found that CPE co-localizes with and interacts with GH in AtT20 pituitary cells. Downregulation of CPE led to decreased levels of GH secretion, consistent with involvement of CPE in GH sorting/secretion. Our binding assay in vitro with bacterially expressed proteins suggested that GH directly interacts with CPE but in a manner different from POMC.
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KEYWORD
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AtT20, carboxypeptidase E, growth hormone, pituitary, secretion
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