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KMID : 0578320170400030195
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Molecules and Cells
2017 Volume.40 No. 3 p.195 ~ p.201
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MiR-186 Inhibited Migration of NSCLC via Targeting cdc42 and Effecting EMT Process
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Dong Ying
Jin Xin Tian
Sun Zhi Qiang
Zhao Yue Ming
Song Xian Jing
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Abstract
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In this study, qRT-PCR was employed to identify that miR-186 expression level in NSCLC tissues are highly associated with lymph node metastasis. In addition, through the application of western blotting, luciferase assay and qRT-PCR, it was found that miR-186 targeted 3′UTR of cdc42 mRNA and down-regulated cdc42 protein level in a post-transcriptional manner. Transwell assay indicated that cdc42 partially reversed the effect of miR-186 mimics. Besides, miR-186 was proved to regulate EMT by influencing biomarkers of this process and cell adhesion ability. Thus, miR-186 is a potential target for NSCLC therapy. miR-186 is proposed to be one of tumor-suppressors and may serve as a therapeutic target in NSCLC treatment.
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KEYWORD
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cdc42, EMT, migration, MiR-186, NSCLC
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