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KMID : 0578320170400080533
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Molecules and Cells
2017 Volume.40 No. 8 p.533 ~ p.541
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Engineering and Application of Zinc Finger Proteins and TALEs for Biomedical Research
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Kim Moon-Soo
Kini Anu Ganesh
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Abstract
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Engineered DNA-binding domains provide a powerful technology for numerous biomedical studies due to their ability to recognize specific DNA sequences. Zinc fingers (ZF) are one of the most common DNA-binding domains and have been extensively studied for a variety of applications, such as gene regulation, genome engineering and diagnostics. Another novel DNA-binding domain known as a transcriptional activator-like effector (TALE) has been more recently discovered, which has a previously undescribed DNA-binding mode. Due to their modular architecture and flexibility, TALEs have been rapidly developed into artificial gene targeting reagents. Here, we describe the methods used to design these DNA-binding proteins and their key applications in biomedical research.
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KEYWORD
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biomedical application, sequence-specific DNA detection, transcriptional activator-like effector, zinc fingers
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