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KMID : 0578320180410070631
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Molecules and Cells
2018 Volume.41 No. 7 p.631 ~ p.638
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Self-Reprogramming of Spermatogonial Stem Cells into Pluripotent Stem Cells without Microenvironment of Feeder Cells
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Lee Seung-Won
Wu Guangming
Choi Na-Young
Lee Hye-Jeong
Bang Jin-Seok
Lee Yu-Kyeong
Lee Min-Seong
Ko Ki-Sung
Scholer Hans R.
Ko Ki-Narm
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Abstract
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Spermatogonial stem cells (SSCs) derived from mouse testis are unipotent in regard of spermatogenesis. Our previous study demonstrated that SSCs can be fully reprogrammed into pluripotent stem cells, so called germline-derived pluripotent stem cells (gPS cells), on feeder cells (mouse embryonic fibroblasts), which supports SSC proliferation and induction of pluripotency. Because of an uncontrollable microenvironment caused by interactions with feeder cells, feeder-based SSC reprogramming is not suitable for elucidation of the selfreprogramming mechanism by which SSCs are converted into pluripotent stem cells. Recently, we have established a Matrigel- based SSC expansion culture system that allows longterm SSC proliferation without mouse embryonic fibroblast support. In this study, we developed a new feeder-free SSC self-reprogramming protocol based on the Matrigel-based culture system. The gPS cells generated using a feeder-free reprogramming system showed pluripotency at the molecular and cellular levels. The differentiation potential of gPS cells was confirmed in vitro and in vivo. Our study shows for the first time that the induction of SSC pluripotency can be achieved without feeder cells. The newly developed feederfree self-reprogramming system could be a useful tool to reveal the mechanism by which unipotent cells are self reprogrammed into pluripotent stem cells.
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KEYWORD
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germline-derived pluripotent stem cells, selfreprogramming, spermatogonial stem cells
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