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KMID : 0578320180410070639
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Molecules and Cells
2018 Volume.41 No. 7 p.639 ~ p.645
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Necroptosis Is a Mechanism of Death in Mouse Induced Hepatocyte-Like Cells Reprogrammed from Mouse Embryonic Fibroblasts
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Lee Yun-Suk
Park Kyung-Mee
Yu Li-Na
Kwak Ho-Hyun
Na Hee-Jun
Kang Kyung-Sun
Woo Heung-Myong
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Abstract
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Liver transplantation is recommended for patients with liver failure, but liver donors are limited. This necessitates the development of artificial livers, and hepatocytes are necessary to develop such artificial livers. Although induced hepatocytelike cells are used in artificial livers, the characteristics of mouse induced hepatocyte-like cells (miHeps) reprogrammed with embryonic fibroblasts have not yet been clarified. Therefore, this study investigated the mechanisms underlying the survival, function, and death of miHeps. miHeps showed decreased cell viability, increased cytotoxicity, decreased hepatic function, and albumin and urea secretion at passage 14. Addition of necrostatin-1 (NEC-1) to miHeps inhibited necrosome formation and reactive oxygen species generation and increased cell survival. However, NEC-1 did not affect the hepatic function of miHeps. These results provide a basis for development of artificial livers using hepatocytes.
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KEYWORD
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artificial liver, mouse induced hepatocyte-like cells, necroptosis, necrostatin-1, necrosome formation
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