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KMID : 0578320200430060581
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Molecules and Cells
2020 Volume.43 No. 6 p.581 ~ p.589
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NELL2 Function in Axon Development of Hippocampal Neurons
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Kim Han-Rae
Kim Dong-Hee
An Ji-Young
Kang Da-Sol
Park Jeong-Woo
Hwang Eun-Mi
Seo Eun-Jin
Jang Il-Ho
Ha Chang-Man
Lee Byung-Ju
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Abstract
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Neurons have multiple dendrites and single axon. This neuronal polarity is gradually established during early processes of neuronal differentiation: generation of multiple neurites (stages 1-2); differentiation (stage 3) and maturation (stages 4-5) of an axon and dendrites. In this study, we demonstrated that the neuron-specific n-glycosylated protein NELL2 is important for neuronal polarization and axon growth using cultured rat embryonic hippocampal neurons. Endogenous NELL2 expression was gradually increased in parallel with the progression of developmental stages of hippocampal neurons, and overexpression of NELL2 stimulated neuronal polarization and axon growth. In line with these results, knockdown of NELL2 expression resulted in deterioration of neuronal development, including inhibition of neuronal development progression, decreased axon growth and increased axon branching. Inhibitor against extracellular signal-regulated kinase (ERK) dramatically inhibited NELL2-induced progression of neuronal development and axon growth. These results suggest that NELL2 is an important regulator for the morphological development for neuronal polarization and axon growth.
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KEYWORD
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axon growth, extracellular signal-regulated kinase, hippocampal neurons, neural epidermal growth factor-like like protein 2, neuronal polarity
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