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KMID : 0578320210440030168
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Molecules and Cells
2021 Volume.44 No. 3 p.168 ~ p.178
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Tsg101 Is Necessary for the Establishment and Maintenance of Mouse Retinal Pigment Epithelial Cell Polarity
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Le Dai
Lim So-Yeon
Min Kwang-Wook
Park Joon-Woo
Kim You-Joung
Ha Tae-Jeong
Moon Kyeong-Hwan
Wagner Kay-Uwe
Kim Jin-Woo
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Abstract
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The retinal pigment epithelium (RPE) forms a monolayer sheet separating the retina and choroid in vertebrate eyes. The polarized nature of RPE is maintained by distributing membrane proteins differentially along apico-basal axis. We found the distributions of these proteins differ in embryonic, post-natal, and mature mouse RPE, suggesting developmental regulation of protein trafficking. Thus, we deleted tumor susceptibility gene 101 (Tsg101), a key component of endosomal sorting complexes required for transport (ESCRT), in embryonic and mature RPE to determine whether ESCRT-mediated endocytic protein trafficking correlated with the establishment and maintenance of RPE polarity. Loss of Tsg101 severely disturbed the polarity of RPE, which forms irregular aggregates exhibiting non-polarized distribution of cell adhesion proteins and activation of epidermal growth factor receptor signaling. These findings suggest that ESCRT-mediated protein trafficking is essential for the development and maintenance of RPE cell polarity.
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KEYWORD
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cell polarity, endosomal sorting complexes required for transport (ESCRT), retinal pigment epithelium (RPE), tumor susceptibility gene 101 (Tsg101)
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