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KMID : 0578320230460080513
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Molecules and Cells
2023 Volume.46 No. 8 p.513 ~ p.525
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Color-Tuning Mechanism of the Lit Form of Orange Carotenoid Protein
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Han Man-Hyuk
Yang Hee-Wook
Yoon Jung-Min
Yvette Villafani
Song Ji-Young
Pan Cheol-Ho
Xiaobo Wen
Cho Young-Moon
Song Ji-Joon
Kim Seung-Joong
Park Youn-Il
Ryu In-Kwang
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Abstract
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Orange carotenoid protein (OCP) of photosynthetic cyanobacteria binds to ketocarotenoids noncovalently and absorbs excess light to protect the host organism from light-induced oxidative damage. Herein, we found that mutating valine 40 in the α3 helix of Gloeocapsa sp. PCC 7513 (GlOCP1) resulted in blue- or red-shifts of 6-20 nm in the absorption maxima of the lit forms. We analyzed the origins of absorption maxima shifts by integrating X-ray crystallography, homology modeling, molecular dynamics simulations, and hybrid quantum mechanics/molecular mechanics calculations. Our analysis suggested that the single residue mutations alter the polar environment surrounding the bound canthaxanthin, thereby modulating the degree of charge transfer in the photoexcited state of the chromophore. Our integrated investigations reveal the mechanism of color adaptation specific to OCPs and suggest a design principle for color-specific photoswitches.
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KEYWORD
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canthaxanthin, color-tuning, excited state charge separation, orange carotenoid protein, photoexcitation, photoswitch
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