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KMID : 0620719960020020130
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Natural Product Sciences
1996 Volume.2 No. 2 p.130 ~ p.136
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Antiviral Effects of Natural Products on the Inhibition of Hepatitis B Virus DNA Replication in 2.2.15 Cell Culture System
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Nam Kung-Woo
Chang Il-Moo
Choi Jae-Sue
Hwang Ki-Jun
Mar Woong-Chon
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Abstract
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Evaluation of plant extracts that might inhibit hepatitis B virus (HBV) replication was performed to find potent anti-HBV agents. Eighty-five species of plants from forty-three families were tested for their anti-HBV activities using HBV-producing HepG2-derived 2.2.15 cells. The anti-HBV activity of plant extracts was measured by slot blot hybridization technique and cytotoxicity was determined by crystal violet staining procedure. All plants were extracted with methanol and the extracts were partitioned into n-hexane, ethyl acetate and aqueous layer. The ethyl acetate fractions of Rhus verniciflua (stem: EC50, 8.2㎍/mlⅰCC50, 9.4㎍/ml), Gastrodia elata $(root:\;EC_{50},\;17.7{\mu}g/ml;\;CC_{50},\;>20{\mu}g/ml)$, Raphanus sativus $(seeds:\;EC_{50},\;17.3{\mu}g/ml;\;CC_{50},\;>20{\mu}g/ml)$, and Angelica gigas (root: EC50, 8.3㎍/mlⅰCC50, 15.6㎍/ml) revealed the anti-HBV activity in 2.2.15 cell culture system and these fractions are under the process of further sequential fractionation by column chromatography to find the active principles against HBV.
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KEYWORD
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Hepatitis B virus (HBV), anti-HBV agent, 2.2.15 cells, slot blot hybridization
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