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KMID : 0620719980040020100
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Natural Product Sciences
1998 Volume.4 No. 2 p.100 ~ p.104
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In Vitro Antimutagenic Effects of Alaternin and Isorubrofusarin Gentiobioside from roasted Cassia tora
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Park, Kun Young
Lee, Hee Jung/Choi, Jea Sue/Jung, Gun Ok
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Abstract
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The antimutagenic activity of a methanol extract of roasted Cassia tora seeds against aflatoxin B₁(AFB₁) was demonstrated with the Salmonella typhimurium assay. The numbers of revertants per plate decreased significantly when this extract was added to the assay system using Salmonella typhimurium TA100 and or TA98. The MeOH extract was then sequentially partitioned with CH₂Cl₂, EtOAc, n-BuOH, and H₂O. The CH₂Cl₂ and n-BuOH fractions possessed antimutagenic activity, but the EtOAc and H₂O fractions were inactive. Both the MeOH extract and its fractions were capable of inhibiting the indirect-acting mutagen AFB₁, suggesting that these fractions may prevent the metabolic activation of AFB₁ or scavenge the electrophilic intermediate capable of inducing mutations. Column chromatography using silica gel yielded pure alaternin from the CH₂Cl₂ fraction, and adenosine and isorubrofusarin gentiobioside from the n-BuOH fraction. Alaternin and isorubrofusarin gentiobioside demonstrated significant antimutagenic activities.
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