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KMID : 0620720000060030147
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Natural Product Sciences
2000 Volume.6 No. 3 p.147 ~ p.150
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Anti - tumor Promoting Activity of Some Malaysian Traditional Vegetable ( Ulam ) Extracts by Immunoblotting Analysis of Raji Cells
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Ali, A . M .
Lajis, N . H ./Mooi, L . Y ./Yih, K . Yih/Norhanom, A . W ./Mat, Saleh K ./Yazid, A . M .
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Abstract
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The extracts of Carica papaya (flower), Barringtonia macrostachya (leaves), Coleus tuberosus (tuber), Mangifera indica (fruit skin) and Eugenia polyantha (leaves) showed strong in vitro anti-tumor promoting activity when assayed using Raji cells (Mooi et al., 1999). The anti-tumor promoting activity of the crude extracts was further analyzed by immunoblotting analysis of Raji cells carrying Epstein-Barr virus genome. The expression of early antigens diffuse (EA-D) and early antigens restricted (EA-R) was determined by performing western blotting of treated Raji cells with human sera of nasopharyngeal carcinoma patients. All the plant extracts were shown to be able to suppress both EA-D and EA-R.
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