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KMID : 0620720030090040299
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Natural Product Sciences
2003 Volume.9 No. 4 p.299 ~ p.303
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Screening of Sterol Biosynthesis Inhibitors from Natural Products Using Recombinant Yeast Carrying Human Lanosterol Synthase
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Sung, Chung Ki
Kim, Eun A/Chu, Yun Ho/Shibuya, Masaaki/Ebizuka, Yutaka
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Abstract
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For the screening of inhibitors of sterol biosynthesis from natural products, a simple and rapid assay method was developed using recombinant yeast carrying human lanosterol synthase, main target of this assay method. Sterol biosynthesis inhibition activity was monitored only by the inhibition of growth of the recombinant yeast. By changing the substrate, this assay method can figure out which step is inhibited in the sterol biosnthesis by the test material. With this assay method total 102 plant samples were screened for their inhibitory activity of sterol biosynthesis in ergosterol (-) medium. For selection of the specific inhibitory materials, 11 plant samples were reassayed in ergosterol (+) medium. After all 5 plant samples, Abutilon avicennae Gaertn. (stem), Alnus japonicasteud. (stem), Amaranthus mangostanus L. (aerial part), aerial part), Philadelphus schrenckii Pupr. (leaf) and Pimpinelia brachycarpa Nakai (aerial part), showed specific inhibitory activity.
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