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KMID : 0620720040100010048
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Natural Product Sciences
2004 Volume.10 No. 1 p.48 ~ p.53
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Apoptosis-Inducing Activity of Galloylglucoses from Juglans mandshurica in Human Promyeloid Leukemic HL-60 Cells
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Min Byung-Sun
Kwon Ok-Kyoung
Park Bo-Young
Kim Young-Ho
Hattori Masao
Joung Hyouk
Lee Hyeong-Kyu
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Abstract
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Two galloyl monosaccharides, 1,2,6-trigalloylglucose (1, TRgG) and 1,2,3,6- tetragalloylglucose (2, TEgG), were isolated from the stem-bark of Juglans mandshurica. Two galloylglucoses showed cytotoxic effects on human promyelocytic leukemia HL-60 cells. In order to elucidate their mechanism of action, we have investigated the flow cytometric analysis after Annexin V-FITC and PI staining, caspase-3 activity, and internucleosomal DNA fragmentation in HL-60 cells. HL-60 cells treated with both compounds 1 and 2 at 150 and 100 μM, respectively, led to a morphological features of apoptosis, such as plasma membrane blebbing and cell shrinkage. TRgG (1) and TEgG (2) increased the percentage of FITC+ and FITC+PI+ cells in flow cytometry after Annexin V-FITC and PI staining. The increase of apoptotic cells was preceded by the activation of caspase-3 reported to play a central role in apoptotic process and inducing internucleosomal DNA fragmentation. TEgG (2) showed to have stronger apoptosis inducing activity in HL-60 cell lines as compared with TRgG (1).
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KEYWORD
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Juglans mandshurica, apoptosis, 1, 2, 6-trigalloylglucose, 1, 2, 3, 6-tetragalloylglucose, HL-60 cells, morphological feature, flow cytometry, caspase-3, DNA fragmentation
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