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KMID : 0620720040100050211
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Natural Product Sciences
2004 Volume.10 No. 5 p.211 ~ p.216
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Citrus unshiu Water Extract Inhibits Trypsin-induced TNF-α and Tryptase Productions by Blocking the ERK Phosphorylation and Trypsin Activity
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Kang Ok-Hwa
Kim Dae-Ki
Lee Young-Mi
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Abstract
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Citrus unshiu (Rutaceae) has long been known as an anti-inflammatory and anti-allergic agent. In the present study, the inhibitory effect of CUWE (Citus unshiu water extract) on the production of TNF-α and tryptase was examined. In addition, a possible mechanism for the inhibition of trypsin-stimulated human leukemic mast cell-1 (HMC- 1 ) activation was determined. To do so, TNF-α production from the HMC-1 cells that were stimulated by trypsin (100 nM) in the presence or absence of CUWE (10, 100, and 100 ㎍/ml) was measured by enzyme-linked immunosorbent assay (ELISA) and reverse transcription-PCR. The tryptase production was evaluated by reverse transcription-PCR. Extracellular signal-regulated kinase (ERK) activation was analyzed by Western blot. Trypsin activity was measured by using Bz-DL-Arg-p-nitroanilide (BAPNA) as substrate. Results showed that the CUWE inhibited production of both TNF-α and tryptase from the trypsin-stimulated HMC-1 in a dose-dependent manner. The CUWE a1so inhibited the ERK phosphorylation and trysin activity. These results indicate that the CUWE had an inhibitory effect on TNF-α and the tryptase productions by blocking the ERK phosphorylation and trypsin activity.
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KEYWORD
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Citrus unshiu, trypsin, tryptase, TNF-α, ERK
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