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KMID : 0620720040100060289
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Natural Product Sciences
2004 Volume.10 No. 6 p.289 ~ p.295
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Protection of NMDA-Induced Neuronal Cell Damage by Methanol Extract of Myristica Fragrans Seeds in Cultured Rat Cerebellar Granule Cells
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Ban Ju-Yeon
Cho Soon-Ock
Kim Ji-Ye
Bang Kyong-Hwan
Seong Nak-Sull
Song Kyung-Sik
Bae Ki-Whan
Seong Yeon-Hee
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Abstract
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Myristica fragrans seed from Myristica fragrans Houtt (Myristicaceae) has various pharmacological activities peripherally and centrally. The present study aims to investigate the effect of the methanol extract of Myristica fragrans seed (MF) on N-methyl-D-aspartate (NMDA)-induced neurotoxicity in primary cultured rat cerebellar granule neuron. MF, over a concentration range of 0.05 to 5㎍/ml, inhibited NMDA (1 mM)- induced neuronal cell death, which was measured by trypan blue exclusion test and 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl-tetrazolium bromide (MTT) assay. MF ( 0.5 ㎍/ml) inhibited glutamate release into medium induced by NMDA (1 mM), which was measured by HPLC. Pretreatrnent of MF ( 0.5 ㎍/ml) inhibited NMDA (1 mM)-induced elevation of cytosolic calcium concentration ([Ca ²+]c), which was measured by a fluorescent dye, Fura 2-AM, and generation of reactive oxygen species (ROS). These results suggest that MF prevents NMDA-induced neuronal cell damage in vitro.
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KEYWORD
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Myristica fragrans seed, Neuroprotection, NMDA, Neurotoxicity, Cerebellar granule cells
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