|
KMID : 0620720050110030119
|
|
Natural Product Sciences
2005 Volume.11 No. 3 p.119 ~ p.122
|
|
|
In vitro Free Radical Scavenging and Hepatoprotective Compound from Sanguisorbae Radix
|
|
An Ren-Bo
Tian Yu-Hua
Oh Hyun-Cheol
Kim Youn-Chul
|
|
|
Abstract
|
|
|
|
In the course of searching for hepatoprotective agents from natural products, four compounds were isolated from the MeOH extract of Sanguisorbae Radix, as guided by their DPPH free radical scavenging activity. The structures were determined as 4,5-dimethoxy-3-hydroxybenzoic acid methyl ester (1), (+)-gallocatechin (2), methyl 6-O-galloyl-β-D-glucopyranoside(3), and pomolic acid 3-O-[α-L-arabinopyranoside]-28-O-[β-D-glucopyranosyl] ester (ziyu-glycoside I) (4). Compounds 2 and 3 showed significant DPPH free radical scavenging effects, exhibiting IC50 values of 11.4 and 13.0㎛respectively. L-Ascorbic acid was used as a positive control and exhibited the IC50 value of 50.3㎛ In evaluation of the hepatoprotective activity of the isolated compounds on drug-induced cytotoxicity, compound 2 showed the significant hepatoprotective effect with the EC50 value of 91.84±11.0μM on tacrine-induced cytotoxicity in Hep G2 cells, while silybin, a positive control, exhibited EC50 value of 122.4±12.5μM.
|
|
|
KEYWORD
|
|
|
Sanguisorbae Radix, (+)-gallocatechin, Hepatoprotective, Hep G2 cells, Free radical scavenger
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
 
|
|