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KMID : 0620720050110040207
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Natural Product Sciences
2005 Volume.11 No. 4 p.207 ~ p.212
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Inhibitory Effect of Tetragonia tetragonoides Water Extract on the Production of and Tryptase in Trypsin-Stimulated Human Mast Cells
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Kang Ok-Hwa
Choi Yeon-A
Park Hye-Jung
Tae Jin
Kang Chon-Sik
Lee Dong-Sung
Kim Ju-Ho
Lee Young-Mi
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Abstract
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Tetragonia tetragonoides (Aizoaceae) has been known as an anti-cancer agent. The activation of proteinase-activated receptor-2 (PAR-2) by trypsin appears to play a role in inflammation. In the present study, we examined the inhibitory effects of Tetragonia tetragonoides water extract (TTWE) on the production of tumor necrosis factor-α (TNF - α) and tryptase in trypsin-stimulated human leukemic mast cells (HMC-1) expressing PAR-2. HMC-1 cells were stimulated with trypsin in the presence or absence of TTWE (10, 100, and 1000 ㎍/ml). The level of TNF - α secretion from HMC-1 cells was measured by enzyme-linked immunosorbent assay (ELISA). TNF - α and tryptase mRNA expression were examined by reverse transcription-PCR. Also, extracellular signal-regulated kinese (ERK) activation was assessed by Western blot analysis. Trypsin activity was measured using the substrate Bz-DL-Arg-p-nitroanilide (BAPNA). It was observed that TNF - α secretion, tryptase mRNA and TNF - α mRNA expression in trypsin-stimulated HMC-1 cells were inhibited by pretreatment of TTWE (1000 ㎍/ml). Furthermore, the pretreatment of TTWE (1000 ㎍/ml) resulted in the reduction of ERK phosphorylation and trypsin activity. These results suggest hat TTWE might have the inhibitory effects on the PAR-2-dependent inflammation processes and it is likely to function as PAR-2 antagonist.
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KEYWORD
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Tetragonia tetragonoides, Aizoaceae, trypsin, mast cells, tryptase, TNF-α, ERK
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