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KMID : 0620720060120010038
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Natural Product Sciences
2006 Volume.12 No. 1 p.38 ~ p.43
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Suppression of Cyclooxygenase-2 Expression in Colonic Epithelial Cells by Ilekudinol B Isolated from Weigela subsessilis
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Park Hye-Jung
Choi Yeon-A
Tae Jin
Kang Chon-Sik
Kim Dae-Ki
Thuong Phuong Thien
Kim Young-Ho
Bae Ki-Hwan
Lee Young-Mi
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Abstract
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Ilekudinol B is one of the flavonoids isolated from Weigela subsessilis (Caprifoliaceae). In the present study, the suppression effect of ilekudinol B on tumor necrosis factor (TNF)-α-induecd cyclooxygenase-2 (COX-2) expression was investigated in human colon epithelial cell line HT-29. Interleukin-8 (IL-8) production and prostaglandin E₂(PGE₂)secretion was measured by enzyme-linked immunosorbent assay (ELISA). COX-2 and nuclear factor (NF)-κB expression were determined by Western blot analysis. Ilekudinol B significantly inhibited secretion of IL-8 and prostaglandin E₂(PGE₂) from the human colon epithelial cell line HT-29 in a concentration-dependent manner. In addition, ilekudinol B remarkably diminished TNF-α-induecd COX-2 expression and NF-κB p65 subunit translocation to the nucleus. In conclusion, our results indicate that ilekudinol B may have anti-inflammatory activity on TNF-α-induecd-depedndent
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KEYWORD
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Weigela subsessilis, ilekudinol B, Caprifoliaceae, cyclooxygenase-2, human colon epithelial cells
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