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KMID : 0620720060120020109
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Natural Product Sciences
2006 Volume.12 No. 2 p.109 ~ p.112
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Antiproliferative Constituents from the Vinegar Treated Small Black Soybean (Glycine max Merr.)
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Oh Chan-Ho
Kim Eun-Jeong
Lee Gyu-Hee
Moon Mi-Kyeong
Cho Moon-Gu
Kim Jong-Hwa
Oh Suk-Heung
Lee Tae-Gyu
Shin Tae-Yong
Kim Dae-Keun
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Abstract
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The chloroform-soluble fraction of the vinegar treated small black soybean [Glycine max (Leguminosae)] showed antiproliferative activity against human myeloid leukemia HL-60 cells, in terms of inhibition of proliferation and induction of apoptosis. Bioassay-guided chromatography of the chloroform-soluble fraction resulted in the isolation of two isoflavonoid compounds, genistein and daidzein, as active principles. Genistein showed more potent antiproliferative effects against HL-60 cells. Treatment of HL-60 cells with genistein induced apoptosis in a dose dependent manner. Apoptosis was judged by the detection of DNA fragmentation by a flow cytometry and the degree of apoptosis was assayed by RT-PCR.
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KEYWORD
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Glycine max, antiproliferative activity, genistein
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