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KMID : 0620720060120040237
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Natural Product Sciences
2006 Volume.12 No. 4 p.237 ~ p.240
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Quantitative Analysis of Paeoniflorin from Paeonia lactiflora Using ¹H-NMR
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Yoo Jong-Su
Song Myoung-Chong
Ahn Eun-Mi
Lee Youn-Hyung
Rho Yeong-Deok
Baek Nam-In
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Abstract
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Paeoniflorin, the major component of the root of Paeonia lactiflora, was quantitatively analyzed using ¹H-NMR spectrometry. The quantity of paeoniflorin was calculated by the ratio of the intensity of the signals (H-9, H-10, H-2’, 6’) to the aldehyde peak of the known amount of internal standard, 2,4,6-trihydroxybenzaldehyde. These results were compared with the conventional HPLC method. The contents of paeoniflorin in P. lactiflora, which were respectively calculated by H-9, H-10, H-2’, 6’ in the ¹H-NMR spectra and HPLC, were determined 2.60±0.07, 2.44±0.09, 2.77±0.12 and 2.46±0.16%. The advantages of quantitative ¹H-NMR analysis are that can be analyzed to identify and quantify, and no reference compounds required for calibration curves. Besides, it allows rapid and simple quantification for paeoniflorin with an analysis time for only 20 min without any preprocessing.
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KEYWORD
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¹H-NMR, quantitative analysis, Paeonia lactiflora, paeoniflorin
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