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KMID : 0620720090150010027
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Natural Product Sciences
2009 Volume.15 No. 1 p.27 ~ p.31
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Melanin-concentrating Hormone-1 Receptor (MCH-1) Antagonism of the Leaves Extract from Morus alba
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Oh Byung-Koo
Oh Kwang-Seok
Lee Sung-Hou
Seo Ho-Won
Choi Yeon-Hee
Choi Jae-Seok
Kim Young-Sup
Lee Byung-Ho
Kwon Kwang-Il
Ryu Shi-Yong
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Abstract
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The present study was performed to investigate the binding affinity of the ethanol extract from the leaves of Morus alba (EMA) and some EMA related plant materials (EMA-D, EMA-DM) for melaninconcentrating hormone-1 receptor (MCH-1) and also to examine the antagonistic effect of them for the recombinant MCH-1 receptor expressed in CHO cells. EMA, dichloromethane fraction (EMA-D) and EMA-DM exhibited high affinity for mammalian MCH receptor in receptor binding assays (IC50 value: 2.3, 1.6 and 1.0 μg/ml, respectively). Other plant materials (MMA-D, MMA-DM) obtained from methanol extracts from the leaves of Morus alba (MMA) also exhibited high affinity for mammalian MCH receptor, even though the IC50 values of them were lower than those of EMA-D and EMA-DM. In Chinese hamster ovary (CHO) cells expressing human MCH-1, EMA-DM and EMA-D significantly inhibited MCH-induced intracellular Ca2+ increase (IC50 values: 16.5 and 22.7 μg/ml, respectively). These results clearly indicate that the ethanol extract from the leaves of Morus alba (EMA) and some EMA related plant materials (EMA-D, EMA-DM) are novel selective MCH-1 receptor antagonist, respectively.
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KEYWORD
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Morus alba, Moraceae, Melanin-concentrating hormone, MCH-1 receptor antagonist
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