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KMID : 0620720090150010050
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Natural Product Sciences
2009 Volume.15 No. 1 p.50 ~ p.54
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Quantitative Determination of Diterpenoids from the Roots of Aralia cordata
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Lee Mi-Kyoung
Hung Tran-Manh
Min Byung-Sun
Lee Ik-Soo
Na Min-Kyun
Woo Mi-Hee
Son Jong-Keun
Kim Young-Ho
Choi Jae-Sue
Bae Ki-Hwan
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Abstract
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A simple and reliable reversed phase HPLC method was developed to determine pharmacologically active compounds ent-continentalic acid, kaurenoic acid, and continentalic acid from the roots of Aralia cordata. Quantitative analysis of diterpenoids of A. cordata were performed by reverse phase C-18 column using a isocratic of acetonitrile : 0.1% trifluoroacetic acid (70 : 30) with UV detection at 205 nm. The correlation coefficients of each calibration curve of ent-continentalic acid, kaurenoic acid, and continentalic acid were 0.9987, 0.9983, and 0.9986, respectively. The detection limit of ent-continentalic acid, kaurenoic acid, and continentalic acid were 0.5~ 1.0 μg/mL, respectively. The contents of ent-continentalic acid (1), kaurenoic acid (2), and continentalic acid (3) of the roots of A. cordata collected from seventeen district markets in Korea were 0.09~0.75 mg/g, 1.09~5.43 mg/g, and 2.69~9.08 mg/g, respectively.
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KEYWORD
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Aralia cordata, HPLC, ent-continentalic acid, kaurenoic acid, continentalic acid
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