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KMID : 0620720100160010039
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Natural Product Sciences
2010 Volume.16 No. 1 p.39 ~ p.42
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Determination of Hyperin in the Fruits of Acanthopanax Species by High Performance Liquid Chromatography
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Lee Jeong-Min
Kim Hye-Min
Lee Sul-Lim
Han Saem
Cho Seon-Haeng
Lee Sang-Hyun
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Abstract
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The content of hyperin in Acanthopanax species was determined by high performance liquid chromatography (HPLC). Hyperin was quantified by a reverse-phase column with elution program [initially gradient solvent (acetonitrile:water=85:15 to 80:20 for 20 min), then isocratic solvent (acetonitrile:water=80:20 for 20 min), and finally gradient solvent (acetonitrile:water=80:20 to 65:35 for 20 min)]. UV detection was conducted at 210 nm. The content of hyperin in the fruits of Acanthopanax was measured in the species A. chiisanensis (2.04 mg/g), A. sessiliflorus (1.13 mg/g), A. divaricatus (0.98 mg/g), A. koreanum (0.75 mg/g) and A. senticosus (0.05 mg/g). The content of hyperin in A. chiisanensis was higher than that of other Acanthopanax species.
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KEYWORD
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Acanthopanax, Araliaceae, quantitative analysis, flavonoid, hyperin
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