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KMID : 0620720100160020068
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Natural Product Sciences
2010 Volume.16 No. 2 p.68 ~ p.74
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Lipoxygenase Inhibitory and Antioxidant Activities of Isolated Compounds from Moutan Cortex
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Ha Do-Thi
Trinh Nam Trung
Yim Nam-Hui
Bae Ki-Hwan
Nguyen Duy Thuan
Min Byung-Sun
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Abstract
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Phytochemical investigation on the ethyl acetate and n-butanol fractions of Moutan Cortex resulted in the isolation and characterization of a new monoterpene glycoside (3) and twenty known monoterpene glycosides (1, 2, 4 -21). The structure of 3 was determined by spectroscopic data interpretation and physico-chemical properties. Compounds 1 and 8 presented a remarkable inhibitory activity against lipoxygenase-1 (LOX-1) with IC50 values of 45.2 and 37.5 ?M, respectively. Compounds 9, 10, 13, 18, 19, and 21 showed significant 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging effect with IC50 values of 9.8, 25.5, 6.4, 15.2, 18.7, and 23.7 ?M, respectively. Benzoylpaeoniflorin (8), which exhibited the highest inhibitory effect with an IC50 value of 37.5 ± 0.7 ?M, was further analyzed the inhibition kinetics by Lineweaver-Burk plots. Results indicated that 8 is a non-competitive inhibitor, and the kinetic parameter values were estimated to be (31.04 ?M, Ki), (0.29 ?M/min, Vm), and (48.50 ?M, Km).
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KEYWORD
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Moutan Cortex, monoterpenes, LOX-1, DPPH
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