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KMID : 0620720100160020088
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Natural Product Sciences
2010 Volume.16 No. 2 p.88 ~ p.92
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Eudesmols Induce Apoptosis through Release of Cytochrome c in HL-60 Cells
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Hoang Duc-Manh
Long He
Kim Bo-Yeon
Ahn Jong-Seog
Trinh Nam Trung
Ha Do-Thi
Bae Ki-Hwan
Lee Myoung-sook
Hoang Van Luong
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Abstract
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We verified that the apoptosis activities were examined by DNA fragmentation, flow cytometric analysis with annexin V staining, activation of caspase-3, and cytochrome c release. In the result, α-and β-eudesmol induced DNA fragmentation in HL-60 cells at a concentration of 80 ?M, respectively. Additionally, pro-apoptotic cells sorted by flow cytometry analysis were detected in HL-60 cells to 31.77 and 29.67% with aand β-eudesmol of 80 ?M. Thus, both α-and β-eudesmol exerted caspase-3 activation and cytochrome c release at 80 ?M in HL-60 cells. These results are firstly reported that the sesquiterpenes, α-and β-eudesmol are apoptosis inducers through mitochondria-dependent caspase cascade in HL-60 cells.
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KEYWORD
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Magnolia obovata, sesquiterpene, eudesmols, apoptosis inducer, phytochemical
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