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KMID : 0620720100160030164
Natural Product Sciences
2010 Volume.16 No. 3 p.164 ~ p.168
Characterization of Triterpenoids, Flavonoids and Phenolic Acids in Eclipta prostrata by High-performance Liquid Chromatography/diode-array Detector/electrospray Ionization with Multi-stage Tandem Mass Spectroscopy
Lee Ki-Yong

Ha Na-Ry
Kim Tae-Bum
Kim Young-Choong
Sung Sang-Hyun
Abstract
High-performance liquid chromatographic method with diode-array detector and electrospray ionization with multi-stage tandem mass spectroscopy (HPLC/DAD/ESI-MSn) was used to identify the major constituents in a methanolic extract of Eclipta prostrata. The chromatographic separation was performed on a C18 column. Acetonitrile-water was used as a mobile phase. HPLC/DAD/ESI-MSn allowed the characterization of constituents of E. prostrata, mainly triterpenoids (eclalbasaponin I, ¥±, ¥², ¥³, ¥µ), flavonoids (luteolin 7-O-glucoside, demethylwedelolactone, wedelolactone, luteolin, demetylwedelolactone sulfate, luteolin sulfate, apigenin sulfate) and phenolic acids (5-O-caffeoylquinic acid, 3, 4-O-dicaffeoylquinic acid, 3, 5-O-dicaffeoylquinic acid, 4, 5-Odicaffeoylquinic acid).
KEYWORD
Eclipta prostrata, HPLC/DAD/ESI-MSn, triterpenoids, flavonoids, phenolic acids
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