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KMID : 0620720100160030180
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Natural Product Sciences
2010 Volume.16 No. 3 p.180 ~ p.184
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Simultaneous Determination of (-)-Menthone and (-)-Menthol in Menthae Herba by Gas Chromatography and Principal Component Analysis
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Kim Jung-Hoon
Seo Chang-Seob
Shin Hyeun-Kyoo
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Abstract
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The simple and accurate method was established for the simultaneous determination of (-)-menthone and (-)-menthol in Menthae herba obtained from Korea and China. A quantitative analysis was performed with a gas chromatography-flame ionization detector and reference compounds were separated on a capillary HP-Innowax column (30m×0.23mm, 0.50㎛, Agilent, MA, USA). The correlation coefficients of the compounds showed good linearity (r2>0.9997) over the linear range. The precision, repeatability and stability showed less than 1.7% of relative standard deviation (RSD) values for two compounds. Recovery rates were within the range of 95.72-103.76%. The method was applied successfully to analyze 15 samples of Menthae herba and achieved sufficient and specific separation of reference compounds. The principal component analysis (PCA) exhibited the classification of 15 samples according to their locations of origin.
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KEYWORD
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Gas chromatography, Menthae herba, -Menthone, -Menthol, Principal component analysis
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