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KMID : 0620720100160040228
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Natural Product Sciences
2010 Volume.16 No. 4 p.228 ~ p.232
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The Isolation of Acetylcholinesterase Inhibitory Constituents from Lycoris radiate using On-Line HPLC-biochemical Detection System
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Yang Hee-Jung
Yoon Kee-Dong
Chin Young-Won
Kim Young-Choong
Kim Jin-Woong
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Abstract
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Bioactivity-guided fractionation using on-line HPLC biochemical detection system on CHCl3-soluble fraction of Lycoris radiata led to the isolation of deoxylycorenine (1), O-demethylhomolycorine (2), galanthamine (3), lycoramine (4), mixture of 6α-and 6β-haemanthidine (5), and lycorine (6), identified by spectroscopic data and physicochemical property. Among the isolated compounds, 1, 3 and 6 showed acetylcholinesterase inhibitiory activities with IC50 values of 18.0, 12.0 and 16.6 μM, respectively, in in vitro colorimetric microplate assay.
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KEYWORD
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Lycoris radiate, Amaryllidaceae, acetylcholinesterase, galanthamine, deoxylycorenine, Lycorine, flow biochemical detection system, on-Line HPLC-biochemical detection system
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