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KMID : 0620720100160040251
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Natural Product Sciences
2010 Volume.16 No. 4 p.251 ~ p.258
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Quantitative Analyses for the Quality Evaluation of Salviae Miltiorrhizae Radix by HPLC
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Zhe Fang
Min Byung-Sun
Woo Mi-Hee
Moon Dong-Cheul
Son Kun-Ho
Son Jong-Keun
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Abstract
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In this study, quantitative analysis for the quality evaluation of Salviae Miltiorrhizae Radix using HPLC/UV was developed. For quantitative analysis, six major bioactive compounds were determined. The separation conditions employed for HPLC/UV were optimized using ODS C18 column (250×4.6 mm, 5 μm) with gradient condition of A (1% formic acid in H2O) and B (acetonitrile: methanol: formic acid=100:75:1) as the mobile phase at a flow rate of 1.0 mL/min and a detection wavelength of 280 nm. These methods were fully validated with respect to the linearity, accuracy, precision and recovery. The HPLC/UV method was applied successfully to the quantification of six major compounds in the Salviae Miltiorrhizae Radix. The results indicate that the established HPLC/UV method is suitable for the quantitative analysis.
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KEYWORD
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Salviae Miltiorrhizae Radix, HPLC, Quality control
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