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KMID : 0620720100160040259
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Natural Product Sciences
2010 Volume.16 No. 4 p.259 ~ p.264
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Effects of the Essential Oil Components from Ligusticum chuanxing on Proinaflammatory Mediators of RAW264.7 Macrophage Cells
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Lim Hye-Rim
Shin Seung-won
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Abstract
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The essential oil fraction was obtained from the underground parts o of Ligusticum chuanxiong (Umbelliferae) by steam distillation, and its main components, Z-ligustilide and butylidene phthalide, were isolated by column chromatography. Its essential oil fraction and the isolated main components were examined for effects on their anti-inflammatory properties in RAW 264.7 macrophage cells to develop a new natural antiinflammatory drug. The results showed that the L. chuanxiong essential oil fraction and its main components, Zligustilide and butylidene phthalide, inhibited the production of nitric oxide significantly in lipopolysaccharide (LPS)-treated RAW 264.7 cells. LPS-induced interleukin-1B (IL-1B), interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-a) production was also decreased in a dose-dependent manner. In addition, western blot analysis revealed that the L. chuanxiong essential oil fraction and also its main components, Z-ligustilide, and butylidene phthalide reduced the expression levels of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS).
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KEYWORD
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Ligusticum chuanxiong, essential oil, Ligustilide, butylidene Phthalide, anti-inflammatory effect, nitric oxide, IL-1B, IL-6, TNF-a, COX-2, iNOS
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