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KMID : 0620720100160040265
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Natural Product Sciences
2010 Volume.16 No. 4 p.265 ~ p.270
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Zanthoxylum rhetsa Stem Bark Extract Inhibits LPS-induced COX-2 and iNOS expression in RAW 264.7 Cells via the NF-kB Inactivation
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Nguyen Bich Thu
Ha Do-Thi
Trinh Nam Trung
Nguyen Minh Khoi
Bae Ki-Hwan
Nguyen Viet Than
Thipthaviphone Soulinho
Nguyen Hai Nam
Tran Thi Phuong
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Abstract
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The methanol extract of Zanthoxylum rhetsa (MZRR) were evaluated for its ability to suppress the formation of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in lipopolysaccharide (LPS)-activated RAW 264.7 macrophages. MZRR presented an inhibition of LPS-induced production of nitric oxide (NO) and prostaglandin E2 (PGE2) in RAW 264.7 macrophages. Western blotting and RT-PCR analyses demonstrated that MZRR significantly inhibited the protein and mRNA expressions of iNOS and COX-2 in LPSactivated macrophages in a dose-dependent manner. LPS-induced COX-2, iNOS, and nuclear factor kappa beta (NF-kB) activity were also decreased in the presence of MZRR. The production of tumor necrosis factor-α (TNF-α), the mRNA expression levels of pro-inflammatory cytokines, including TNF-α and IL-1β, were reduced after MZRR administration in a dose dependent-manner. These results suggest that the MZRR extract involved in the inhibition of iNOS and COX-2 via the NF-κB pathway, revealing a partial molecular basis for anti-inflammatory properties of the MZRR extract.
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KEYWORD
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Zanthoxylum rhetsa, COX-2, iNOS, NF-kB, RAW 264.7
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