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KMID : 0620720110170020104
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Natural Product Sciences
2011 Volume.17 No. 2 p.104 ~ p.107
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A Convenient HPLC/ELSD Method for the Quantitative Analysis of Betaine in Lycium chinense
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Lee Sang-Myung
Park Chae-Kyu
Cho Byung-Goo
Cho Kyoung-Shim
Min Byung-Sun
Bae Ki-Hwan
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Abstract
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In order to facilitate the quality control of betaine from the fruits of Lycium chinense, we have developed a rapid and simple method for quantitative determination. Determination was achieved on a Discovery C18 column with an isocratic solvent system of 0.32% perfluoropentanoic acid aqueous-acetonitrile at a flow-rate of 0.5 mL/min and detected an ELSD. The method was reproducible with intra- and inter-day variations of less than 6% (R.S.D). The recoveries were in the range of 90.01~100.05%. The method turned out to be fast and simple, furthermore, to have a good selectivity and sensitivity for the quantity determination of betaine in the fruits of L.chinense.
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KEYWORD
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Lycium chinense, Solanaceae, Betaine, HPLC-ELSD detector
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